Search found 6 matches

by sahart
Wed Jan 19, 2011 9:32 am
Forum: Molecular Biology
Topic: promoter location
Replies: 5
Views: 4056

Re:

well, it's upstream of your gene, so if you read your gene in reversed complement, than yes, downstream (but there's no need to do it that way. How do you know, which strand is normal and which reversed?) well I found this information in ensemble website. it mentioned this gene is reverse strand. W...
by sahart
Wed Jan 19, 2011 3:11 am
Forum: Molecular Biology
Topic: promoter location
Replies: 5
Views: 4056

Re:

Upstream of your gene. Sequence from the gene down and see what does have an effect. Or if you have the sequence available, try to amplify and clone the sequence between your gene, and the one before. You can even make multiple slices to identify which part are relevant. Clone in front of gene that...
by sahart
Tue Jan 18, 2011 4:12 am
Forum: Molecular Biology
Topic: promoter location
Replies: 5
Views: 4056

promoter location

Greetings,

I am going to clone the promoter of a zebrafish gene. Do you have any idea that how I should find its location?

Thank you :)

Sahar
by sahart
Tue Jan 18, 2011 4:11 am
Forum: Molecular Biology
Topic: How can I extend my sequencing results?
Replies: 8
Views: 3287

Re:

canalon wrote:Sequence walking: Design a new primer at the end of the one you have.


How much should I add cDNA as template?
by sahart
Tue Jan 18, 2011 4:09 am
Forum: Molecular Biology
Topic: How can I extend my sequencing results?
Replies: 8
Views: 3287

Thanks all
by sahart
Mon Jan 17, 2011 12:24 pm
Forum: Molecular Biology
Topic: How can I extend my sequencing results?
Replies: 8
Views: 3287

How can I extend my sequencing results?

Hi all,

I have sequenced around 400 bp of a cDNA, I am wondering how can i extend my sequencing result to whole the cDNA, kindly noted that I have no information about other part of this mRNA.

Thank you, :)

Sahar