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by karthikmgt
Sat Oct 16, 2010 11:27 am
Forum: Molecular Biology
Topic: cloning of gene in pcr4-topo vector or PQE 30 not successful
Replies: 1
Views: 3875

Re: cloning of gene in pcr4-topo vector or PQE 30 not successful

hi there...

did u add IPTG when you spread plated your transformation reaction mix.... bcos pCR4 TOPO has lac promoter that governs the entire vectors genes(amp,kan) and in dh5alpha lac repressor is active... so i think if you add IPTG and spread plate you will get colonies.....