Search found 13 matches

by swing0916
Thu Nov 11, 2010 10:52 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

Blue light can excite EtBr absolutely. Many people knows. All the issues are ONLY about contrast.
by swing0916
Thu Nov 11, 2010 10:49 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

It is hard to say. From the spectrum of EtBr, it shows the response of 302 nm is approximately 5 times higher than that of 500 nm. For consideration of intensity of lights, UV lamptube is with diverse light but LED is with a more intense and focusing light. It means the convergence of light cone ang...
by swing0916
Wed Nov 10, 2010 3:46 pm
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

If you highly doubt about excitation at 500 nm and emission at 600 nm with a filter to cut 500 nm background to see the bands, you can simply check the mechanism of Sybr dyes. The difference between excitation and emission wavelength of SyBrSafe is even smaller. So it is possible for EtBr to excite ...
by swing0916
Wed Nov 10, 2010 7:28 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

Re: UltraBright UV transilluminator

From the attached EtBr spectrum, it is possible to excite EtBr by VIS light, expecially blue wavelength. Band image relates to contrast. If intensity of blue light is high enough, the contrast of band image can be acceptable. We cannot say ONLY UV can be used for EtBr.
by swing0916
Wed Nov 10, 2010 4:55 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

Many researchers told me in US this instrument of UltraBright LED transilluminator is quite different from others like SafeImager or DarkReader and they don't like to wear amber glasses to cut gel. Canalon said the difference is minimal. I am interested in these different opinions.
by swing0916
Mon Nov 08, 2010 2:29 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

There are not so many LED transilluminators in market. I think LED visible light is much safer than UV. This is a video I found yesterday about [some] LED transilluminator.
http://www.youtube.com/watch?v=mjqBlxcQBds
by swing0916
Fri Oct 22, 2010 2:28 pm
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

If you look at EtBr's excitation and emission spectrum, it is found the absorption is strongest near 300 nm. It is why most people use 302 nm UV to illuminate EtBr and cut bands. But you can see there is also absorption at 470~500 nm of EtBr which means if the intensity is high enough, the fluoresce...
by swing0916
Wed Oct 20, 2010 6:17 am
Forum: Molecular Biology
Topic: Ethidium Bromide (EtBr) or safe dyes???
Replies: 3
Views: 2687

Even if US, there is still 70 % using EtBr. Is your lab using EtBr or safe dyes?? Why?
by swing0916
Wed Oct 20, 2010 6:15 am
Forum: Molecular Biology
Topic: Ethidium Bromide (EtBr) or safe dyes???
Replies: 3
Views: 2687

I am interested in safe dyes, but people do care about cost. We cannot afford to these safe dyes yet. Maybe it is a trend in the future that safe dyes price will be more acceptable for most of the labs to use.
by swing0916
Mon Oct 18, 2010 11:48 pm
Forum: Molecular Biology
Topic: Ethidium Bromide (EtBr) or safe dyes???
Replies: 3
Views: 2687

Ethidium Bromide (EtBr) or safe dyes???

Most people use Ethidium Bromide (EtBr) as a fluorescent dye for DNA. People know it is poisonous and dangerous to cause cancer. Also people use UV to excite EtBr, which is risky for eyes and skins. Some people start to use safe dyes, like SyBr Safe, Gelgreen, Gelred, etc. They are safer but more ex...
by swing0916
Sat Oct 16, 2010 1:31 pm
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

Re: UltraBright UV transilluminator

I used a photospectrometer to check the spectrum at UV lamp and black glass positions, and found there is visible light emitted from the lamps. When the lights go through the black glass filter, there is nearly only UV rays transmitted. I guess it's why the image of DNA bands is with a better contra...
by swing0916
Fri Oct 15, 2010 11:49 am
Forum: Molecular Biology
Topic: UltraBright UV transilluminator
Replies: 17
Views: 21257

Re:

what you mean with the lamp on? I mean when turn on the power of UV transilluminator, the light-on lamp of 302 nm or 365 nm cannot be seen. Normally, I will see the UV lamp when light on for most of the UV transilluminators, including the old one in our lab. There is a picture I found on their webs...