Search found 13 matches

by NiceOnTheIce
Wed Mar 09, 2011 9:12 pm
Forum: Molecular Biology
Topic: Cytochrome C smear in SDS-PAGE
Replies: 3
Views: 2804

Thanks for the response, canalon. Cytochrome C degradation makes sense seeing as the smear started where the band should have been, and continued past it. Could you explain to me how degradation might happen?
by NiceOnTheIce
Wed Mar 09, 2011 6:23 pm
Forum: Molecular Biology
Topic: Cytochrome C smear in SDS-PAGE
Replies: 3
Views: 2804

Cytochrome C smear in SDS-PAGE

I ran an SDS-PAGE for my lab, and in two of the wells I loaded a Cytochrome C/buffer mixture. Both of these lanes resulted in a light smear starting at approx halfway down the gel and ending approx 3/4 of the way down the gel. What would be the reason for the smear? Note: All of my other protein ban...
by NiceOnTheIce
Fri Jan 14, 2011 2:43 am
Forum: Genetics
Topic: Punnett Square Question
Replies: 5
Views: 3421

Yes, exactly. We have learned about the variations to Mendel's principles, but they aren't being applied in this first assignment. Only the basic "Mendelian" should be used in this case. What I thought was, since my prof said that the 7 F1 offspring (4 black:3 pink) was a 1:1 ratio (crossi...
by NiceOnTheIce
Thu Jan 13, 2011 11:35 pm
Forum: Genetics
Topic: Punnett Square Question
Replies: 5
Views: 3421

There isn't any more information, but I'm pretty sure this assignment doesn't cover the variations to Mendel's Principles. Just the basic, "pea plant" scenarios. (I'd thought of lethal genes too.)
by NiceOnTheIce
Thu Jan 13, 2011 4:35 pm
Forum: Genetics
Topic: Punnett Square Question
Replies: 5
Views: 3421

Punnett Square Question

In rats, a black-tailed mutant was discovered. When it was crossed to a pink tailed rat, 4 offspring were black-tailed and 3 were pink tailed. Two of these black-tailed rats were crossed and they produce 6 black tailed and 3 pink-tailed rats. These experiments were repeated several times with approx...
by NiceOnTheIce
Sun Nov 28, 2010 6:31 pm
Forum: Molecular Biology
Topic: Lambda DNA stuck in well
Replies: 2
Views: 2800

Lambda DNA stuck in well

Hi there, Why might my uncut lambda DNA have gotten stuck in my agarose gel well? When I had the gel visualized and a picture printed of my fluorescing bands, I noticed that my uncut lambda DNA had not moved from the well, except for a light band that did not even travel as far as the band depicting...
by NiceOnTheIce
Sun Nov 21, 2010 11:27 pm
Forum: Molecular Biology
Topic: Inserting DNA Fragment into Plasmid
Replies: 3
Views: 7245

Re: Inserting DNA Fragment into Plasmid

The full question is this: "You are a summer research student in a molecular biology research lab. You are asked to insert the "stresslessontests" gene into a plasmid. It is important that the gene is inserted in the proper orientation so that a functional protein can eventually be ma...
by NiceOnTheIce
Sun Nov 21, 2010 1:53 am
Forum: Molecular Biology
Topic: Inserting DNA Fragment into Plasmid
Replies: 3
Views: 7245

Inserting DNA Fragment into Plasmid

Scenario: You digest DNA into fragments and cut plasmids at a specific site, then mix them together and add ligase to hopefully insert a DNA fragment into a plasmid and close it up. How do you ensure that the DNA is inserted in the proper orientation (not backwards?) Assume the DNA has sticky ends a...
by NiceOnTheIce
Thu Nov 18, 2010 12:40 pm
Forum: Cell Biology
Topic: Resuspending Cells
Replies: 4
Views: 14249

That makes sense, but we were specifically told that we would not be using glass pipettes (just estimating the amount of CaCl2 according to the lines on our centrifuge tube) and that vortexing would kill our cells. So I still don't know what to do.
by NiceOnTheIce
Wed Nov 17, 2010 5:38 pm
Forum: Cell Biology
Topic: Resuspending Cells
Replies: 4
Views: 14249

Resuspending Cells

For my lab tomorrow there's a step that says: "Add ~2mL of ice cold 0.1M CaCl2 to the cell pellet and gently resuspend the cells." I know what resuspending means but I don't know how to go about doing this, as we are supposed to pour the CaCl2 straight into the tube (approx ~2mL) and can't...
by NiceOnTheIce
Thu Nov 04, 2010 11:28 pm
Forum: Molecular Biology
Topic: Properties of Amino Acid Side Chains
Replies: 1
Views: 3398

Properties of Amino Acid Side Chains

Hi there, I've memorized the names and the one letter codes for all 20 amino acids, but would someone be able to tell me the properties (hydrophobic, polar, or ~neutral) of all the side chains? Every web site I go to says something different, and my professor didn't add that to his slides. I know th...
by NiceOnTheIce
Thu Oct 14, 2010 1:19 am
Forum: Microbiology
Topic: Inoculating bacteria
Replies: 5
Views: 6771

Re: Inoculating bacteria

That makes sense.
But continuing on from that point: Does each colony of bacteria differ from one another? Could that be another way that the culture would become "contaminated" by using 2 or 3 colonies? Or are the colonies pretty much identical?
Thanks.