Search found 28 matches

by Nmr
Tue Nov 15, 2011 3:42 pm
Forum: Molecular Biology
Topic: Primer dimer
Replies: 1
Views: 2459

Primer dimer

Hi guys.. I wish that u can help me to come over this problem ! I had done PCR for 16s rRNA gene using colony PCR technique and i used and then i sent my pcr products to the 1st base company without purification in order to do sequencing ... they sent me back and they said that i have primer dimer w...
by Nmr
Sat Sep 17, 2011 2:14 pm
Forum: Microbiology
Topic: ANNTI FUNGI !
Replies: 5
Views: 4031

ANNTI FUNGI !

HI GUYS...

WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?

COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !

THANK YOU
by Nmr
Sat Sep 17, 2011 6:24 am
Forum: Microbiology
Topic: Halide assay
Replies: 1
Views: 2791

Halide assay

Hi guys.. how are you doing ? i wish doing well !

Here, i have a quick question about Halide assay ...How can i measure the chloride gas from bacterial sample using Gas Chromatography ? and what is the procedure to prepare the standard value ?

Thank you
by Nmr
Sun Sep 04, 2011 2:37 am
Forum: Microbiology
Topic: the flavors of our life !
Replies: 0
Views: 2189

the flavors of our life !

Hi again guy.... after I had a very long vocation I went back to my lab to continue my project...! but live without problems can not be consider life, so the problems are the flavors of our life and the solution of the problems is the sweet of our life...! this is my vision in life...anyway now and ...
by Nmr
Sun Jul 24, 2011 10:44 am
Forum: General Discussion
Topic: How can i do it ?
Replies: 3
Views: 2372

How can i do it ?

Hi to you all....

in brief.. i want to use 1 mMole of DCA,,but the problem that i have DCA in liquid form which 98% acidity and i want to prepare 10 ml of DCA solution using D.water.
So my question... How i can prepare DCA solution (1 Mole) in order to use it in my medium ?

thanks
by Nmr
Sat Jul 09, 2011 5:23 am
Forum: Microbiology
Topic: It is going to kill me
Replies: 7
Views: 4373

Thank you my friend for your tips and clues...and i will keep trying hopefully to get something .
by Nmr
Thu Jul 07, 2011 10:51 am
Forum: Microbiology
Topic: It is going to kill me
Replies: 7
Views: 4373

Hello guys again... the problem that i have to isolate bacteria from the soil that i believed it is contaminated with Chloroaliphatic compounds such as MCA, DCA, TCA. so i have been preparing the media since 3 weeks ago and i got nothing. until now i prepared 3 different forms of minimal media 2 bro...
by Nmr
Sun Jul 03, 2011 4:24 pm
Forum: Microbiology
Topic: It is going to kill me
Replies: 7
Views: 4373

actually... i dont know the strain of the bacteria. I'm going to identify it.

I have a sample from soil and this soil supposed to have bacteria that able to degrade the MCA which is only the carbon source.
by Nmr
Sun Jul 03, 2011 7:40 am
Forum: Microbiology
Topic: It is going to kill me
Replies: 7
Views: 4373

It is going to kill me

Hi friends...
It is going to kill me....my bacteria not grown yet.. with my minimal media that i prepared using MCA as a carbon source..

any suggestions from you guys that may help me to find out the problem..

thanks
by Nmr
Wed Jun 08, 2011 8:07 am
Forum: Microbiology
Topic: Quickly
Replies: 3
Views: 2085

Guys... If someone has any medium recipe for isolation bacteria,,,?
by Nmr
Wed Jun 08, 2011 7:53 am
Forum: Microbiology
Topic: Quickly
Replies: 3
Views: 2085

Quickly

Hi Friends....

Quick question..how to prepare metal salt solution and basal salt solution and how can i make them ten fold?

thanx
by Nmr
Wed Jun 01, 2011 5:16 pm
Forum: General Discussion
Topic: what is the name of this...!
Replies: 2
Views: 2960

what is the name of this...!

Hi dear friends...

first i wish a very good day for all of you.
seconds i am seeking your help....what is the name of this compound ((NH4)2SO4 ยท 7H2O) and how can i get the Cas No for it in order to order the compound from any supplier company

Best regards...