Search found 3 matches

by shekar
Sat Sep 25, 2010 10:02 am
Forum: Cell Biology
Topic: Cell culture - no CO2
Replies: 10
Views: 36473

mainly co2 role is maintenance of pH for groing of cells if you wont suply the co2 the pH of the cell culture medium will chainge in to basic condition(means pH of the media increase ) maximum cells will grow at pH 6.0 to7.4 , so to salve your problem first you can adjust the pH of the medium in whi...
by shekar
Fri Sep 24, 2010 10:04 am
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 8
Views: 33371

First you need to know preparation of 0.5m EDTA solution Add 186.1 g EDTA (disodium, dihydrate, ) to 800 ml of ddH20. Add about 20g of NaOH pellets while stirring to bring the pH to 8.0. Add the last few grams slowly to avoid overshooting the pH. Note that the EDTA won't completely dissolve until th...
by shekar
Fri Sep 24, 2010 9:23 am
Forum: Molecular Biology
Topic: primer designing
Replies: 4
Views: 4411

primer designing

dear sir/madem

i am working in molecular biology lab ,i need to know designing primers for a specific gene, hole genome, please let me know the process , online sites & need hints,I have whole genome(DNA) sequence ,

thank you