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I need to measure the amount of Serotonin (5-HT) in rat brain cells. In order to prevent degradation of 5-HT, I want to add MAOI into the cell lysate. Is there any supplier for MAOI? What is the suggested amount? Other than MAOI, I also add protease inhibitor cocktail. Is there anymore chemicals to ...
- Fri Sep 24, 2010 1:43 am
- Forum: Molecular Biology
- Topic: The right temperature for unfolding RNA secondary structure
- Replies: 3
- Views: 2458
I think the RNA will re-fold when the temp drops, but the folding may not be the same as the original one as that folding is a random process outside cells.