Search found 160 matches

by Jelanen
Tue Mar 20, 2007 1:51 am
Forum: General Discussion
Topic: Rate of Respiration ... ?
Replies: 6
Views: 3308

CO2 through blood pH. Lower pH means a greater amount of dissolved CO2 (as carbonic acid) so triggers an increase in respiration.
by Jelanen
Tue Mar 20, 2007 1:25 am
Forum: Molecular Biology
Topic: alcoholic fermentation
Replies: 2
Views: 2561

That and you can only get so much alcohol into the solution. At a particular point (a brewmaster knows this stuff) you just can't get a higher concentration of ethanol since it becomes toxic to the yeast.
by Jelanen
Tue Mar 20, 2007 1:22 am
Forum: Molecular Biology
Topic: Please explain to me - CMVp
Replies: 4
Views: 6178

So you can have an inducible promoter for expression in eukaryotic cells.
by Jelanen
Wed Mar 14, 2007 11:43 pm
Forum: Molecular Biology
Topic: Elimination of low-range co-purified proteins
Replies: 3
Views: 2366

If you're running a SDS-PAGE column why are your smaller proteins even still around? They should be long gone by the time a 100kDa fraction comes out. Honestly, I'd start over and put an affinity tag on the protein and use affinity columns.
by Jelanen
Wed Mar 14, 2007 11:31 pm
Forum: Molecular Biology
Topic: expression vector fusion proteins with Ig Fc parts
Replies: 2
Views: 1922

Well, I guess you can start with Invitrogen...
by Jelanen
Sun Mar 11, 2007 8:55 pm
Forum: Evolution
Topic: Evolution - Natural Selection FLAWED?
Replies: 9
Views: 29862

Re: Flawed theory?

However, you need go no further than pathogens that have evolved resistance to antibiotics and insects to insecticides and weeds to weedicide. You only have to look at mutating viral pathogens such as HIV or influenza. We are still cringing in fear about a cross-species avian flu that now infects h...
by Jelanen
Sun Mar 11, 2007 6:40 pm
Forum: Molecular Biology
Topic: E.coli replication time
Replies: 4
Views: 11864

Ohhh, yeah...forgot about that little tidbit when I threw out my first answer. You bacteria people and your tricksy pets.....
by Jelanen
Sun Mar 11, 2007 6:19 pm
Forum: Cell Biology
Topic: Help about DNA replication
Replies: 5
Views: 2478

Specifically DNA dependent DNA polymerase. There are other things also called polymerases or DNA polymerases that have nothing to do with DNA replication, so best to be precise.
by Jelanen
Fri Mar 09, 2007 11:13 pm
Forum: Molecular Biology
Topic: E.coli replication time
Replies: 4
Views: 11864

Not all E. coli strains replicate at 20mins. Lab strains that have been passed too many times or strains like DH5a that are fairly stripped down will have shorter generation times. Or so I've heard.... 8)
by Jelanen
Tue Jul 18, 2006 10:05 pm
Forum: Genetics
Topic: biology question about chromosomes
Replies: 10
Views: 12944

Some organisms use the presence or lack of a chromosome to determine sex. I'm sitting at work apart from my texts (and I don't deal with this stuff on a daily basis anymore *sigh*) but I seem to recall drosophila determine sex this way...someone correct me or something here...
by Jelanen
Mon Apr 17, 2006 2:20 am
Forum: Cell Biology
Topic: Translation
Replies: 2
Views: 2749

well...rough ER is rough due to the ribosomes embedded in the membrane....
HINT HINT
by Jelanen
Sat Apr 15, 2006 11:56 am
Forum: General Discussion
Topic: who's your favourite biologist?
Replies: 24
Views: 15188

no, Karry Mullis used E.Coli polymerase for his PCR experiments. It was Saiki later in 1988 who used thermostable poymerases here and made PCR a revolution Ok, you might get a softer landing from other people on this one, but this is one of my pet peeves. Not so much that you corrected me(happened ...