Search found 5720 matches

by MrMistery
Sun May 30, 2010 4:36 pm
Forum: Molecular Biology
Topic: Enzyme kinetics
Replies: 2
Views: 2545

the graph is showing the reaction rate in your test system. there is definitely more than one enzyme molecule there.

at Vmax virtually all enzymes are occupied with substrate molecules. As soon as one molecule of substrate dissociates, another one binds
by MrMistery
Sun May 30, 2010 1:07 pm
Forum: Cell Biology
Topic: Do pseudounipolar neurons have one axon and one dendrite?
Replies: 2
Views: 7963

the way I learned it is like this. A pseudounipolar neuron has one axon that divides in two. the part before the cell body is functionally a dendrite and structurally an axon. If you really want to classify it you probably want to consider it an axon, since it has myelin and it conducts action poten...
by MrMistery
Mon Apr 19, 2010 1:15 am
Forum: Molecular Biology
Topic: Loading control for different human tissue samples
Replies: 1
Views: 2416

So wait someone else ran a gel and blotted it, and then just sent you the membrane? Jesus, what would they think of next?
Anyway, maybe PGK? Actin?
by MrMistery
Mon Apr 19, 2010 1:08 am
Forum: Molecular Biology
Topic: sample preparation for SDS-PAGE
Replies: 2
Views: 2622

i think this laemmli buffer is just a type of cell lysis buffer. i looked at the recipe and it seems very similar to the sample buffer i use. I mean, BME, glycerol, SDS, bromphenol blue... looks like sample buffer to me
by MrMistery
Mon Apr 19, 2010 12:51 am
Forum: Molecular Biology
Topic: Fusion protein from PCR - construct is reversed.
Replies: 6
Views: 3642

can you post your exact protocol here so I can get a better picture?
by MrMistery
Mon Apr 19, 2010 12:47 am
Forum: Cell Biology
Topic: Smooth endoplasmic reticulum
Replies: 4
Views: 9209

i don't think fast growing cells have an enlarged smooth ER..
Though hepatocytes and myocytes are good examples
by MrMistery
Mon Apr 19, 2010 12:42 am
Forum: Cell Biology
Topic: plasma membrane & protein orientation
Replies: 4
Views: 6110

jackbean is right. the orientation of a protein is established when it is inserted into the membrane of the ER, and can never be changed
by MrMistery
Wed Apr 14, 2010 2:49 am
Forum: Cell Biology
Topic: Cetylpyridinium chloride solution storage
Replies: 4
Views: 4253

well pyridine is degraded by bacteria, that's why i said that you should keep it sterile. But i don't think it would chemically degrade in water (you have it in an aqueous solution right?). though myself i don't like to keep solutions for longer than a few months (that does happen, I sometimes keep ...
by MrMistery
Tue Apr 13, 2010 7:08 pm
Forum: Cell Biology
Topic: Cetylpyridinium chloride solution storage
Replies: 4
Views: 4253

if it was sterile, then it should probably be fine. Just try it and see if it works
by MrMistery
Mon Apr 12, 2010 11:51 pm
Forum: Cell Biology
Topic: p53 mutation help?
Replies: 10
Views: 7684

i suppose technically you're right - if you wanna be specific loss of heterozygosity means that you would end up with two identical bad alleles, and the only way that would happen would be recombination.
by MrMistery
Mon Apr 12, 2010 12:03 am
Forum: Botany Discussion
Topic: wrap flower with green cellophane
Replies: 4
Views: 4037

I think this is not the case. There is a difference between how a plant sees light for photosynthesis and how a plant sees light for information purposes. for photosynthesis, a plant sees light with chlorophyll, and accessory pigments such as phycobillins (carotenoids are sometimes listed as accesso...
by MrMistery
Sun Apr 11, 2010 11:11 pm
Forum: Molecular Biology
Topic: JackBean's Concanavalin A
Replies: 3
Views: 2671

wait, you solved the structure of Concanavalin A?