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- Fri Sep 10, 2010 8:44 pm
- Forum: Molecular Biology
- Topic: How to determine success of a digest producing short segment
- Replies: 4
- Views: 3541
Hi All, I have a plasmid of 4.008 kb which I have digested with BamHI and NotI concurrently. The digestion is supposed to produce two segments of of 45 bp and 3953 bp respectively. I ran the digestion on 1.5% gel but there was no obvious difference between the linearised vector and the doubly digest...