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Thank you. You are right. Since the interference is by binding of antisense RNA strand of 21-24 nt in RISC to mRNA, if introns are present they will not have complementary sequence in mRNA. In short, there is no short cut. It has to be cDNA.
In plants for getting a hairpin loop structure in pHannibal vector people normally use cDNA as the starting material from which to amplify the gene of interest. My question is whether one can start with the genomic DNA? What happens if introns are present in the region which will form ds RNA after t...