Search found 6 matches

by biostud
Tue May 10, 2011 8:50 pm
Forum: Cell Biology
Topic: Cell mag access
Replies: 0
Views: 891

Cell mag access

Guys, i need your help! I am working on a project. It is about an article i downloaded from the net 2 weeks ago, when my library had access on cell.com but now it doesnt anymore. So i am looking for someone who has access and can download the supplementary information of this article! Please, can an...
by biostud
Sun Jun 27, 2010 9:19 am
Forum: Molecular Biology
Topic: Dna replication initiation point
Replies: 1
Views: 1425

Dna replication initiation point

We all know that identifying DNA initiation points is very difficult! I have somewhere read that there are 3 characteristics of these initiation points! Firstly they contain multiple residues of T/A , secondly the DNA at this point is not condessed (euchromatin) and which is the third one?
by biostud
Fri Jun 25, 2010 8:40 pm
Forum: Molecular Biology
Topic: RNA processing
Replies: 3
Views: 3798

RNA processing

We know that 5' capping and 3' polyadenylation are necessary for the mRNA in eucaryotes. These transformations do not happen in procaryotes. Which is the advantage in eucaryotes, excenp from the fact that the RNA is more protected from exonucleases?
by biostud
Fri Jun 25, 2010 7:58 pm
Forum: Molecular Biology
Topic: Experiment for replication
Replies: 2
Views: 1892

Experiment for replication

How can we experimentally prove that the DNA replication follows 2 directions from one initiation point? In Albert's biology is mentioned an experiment with H^3 thymidine culture of cells, and then after autoradiography we can observe the DNA replication bubble. Is this the experiment?
by biostud
Fri Jun 25, 2010 3:05 pm
Forum: Genetics
Topic: A simple problem
Replies: 3
Views: 2229

A simple problem

Well, we know that during DNA replication there is 1 mistake per 10^9 bases per generation. Well lets suppose we have a gene that contains 10^3 bases. In how many generations there will be one mutation? I think in 10^6 generations! Am i correct? :?:
by biostud
Fri Jun 25, 2010 12:09 pm
Forum: Molecular Biology
Topic: Why polyadenylation?
Replies: 1
Views: 1476

Why polyadenylation?

Why is polyadenylation needed? I know it is necessary to protect the mRNA from nucleases, but i've read that it is also necessary during nuclear export and translation! Why? In what mechanisms does it participate?