Search found 6 matches

by justcurious91
Wed Oct 06, 2010 8:34 pm
Forum: Cell Biology
Topic: functional difference between 70S and 80S RNA
Replies: 1
Views: 1359

functional difference between 70S and 80S RNA

RNA in both eukaryotes and prokaryotes have a similar function of synthesizing proteins and the mechanism for synthesis, elongation and proof reading is also similar. so why is there a difference in their sedimentation number? ( 80S for eukaryotes and 70S for prokaryotes).
by justcurious91
Wed Sep 29, 2010 5:06 am
Forum: Molecular Biology
Topic: RNase digestion problems
Replies: 5
Views: 3631

Re: RNase digestion problems

maybe DNase contamination in the RNase since the DNA is also being digested. so right now i am preparing a fresh stock of RNase.
by justcurious91
Mon Sep 27, 2010 7:50 pm
Forum: Molecular Biology
Topic: RNase digestion problems
Replies: 5
Views: 3631

Re: RNase digestion problems

ya i ran a few controls and observed that everything, even the standards when treated with RNase were showing up as a blank. i think there is some contamination in the RNase but can you explain why the entire lane is appearing as a blank?
by justcurious91
Mon Sep 27, 2010 2:46 am
Forum: Molecular Biology
Topic: RNase digestion problems
Replies: 5
Views: 3631

RNase digestion problems

hi i have run into a few problems in the experiment for plasmid extraction. i did all the steps for boiling lysis followed by phenol-chloroform protocol. for two tubes,i also gave an additional RNase digestion (incubation time 1hr _at_ 37C). during electrophoresis only the samples with phenol-chloro...
by justcurious91
Fri Jun 18, 2010 4:14 am
Forum: General Discussion
Topic: semester long projects any suggestions????
Replies: 3
Views: 4675

ya thats what i think but i dont think they will allow me to do anything much since i have just had 3 full lab courses and most were just titrations and cultures and such. i mean most of this stuff is used by people doing their phd. i wanted to do a project in bio chem related to drug interactions i...
by justcurious91
Thu Jun 17, 2010 6:00 pm
Forum: General Discussion
Topic: semester long projects any suggestions????
Replies: 3
Views: 4675

semester long projects any suggestions????

hi i want to do a semester long project in biology during the fall sem but i can't up with any ideas. can anybody help me out here? P.S. here is a list of all the equipment in the labs at my college:(if that is of any help) Fluorescence Microscope Culture transfer room Bacteriological Incubators Fer...