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by frz
Thu May 06, 2010 5:16 pm
Forum: Molecular Biology
Topic: Help - slow growing lymphoblasts in suspension -not clumping
Replies: 0
Views: 1391

Help - slow growing lymphoblasts in suspension -not clumping

Hi, I was hoping someone could help me. I've been culturing lymphoblasts derived from another center to harvest and make chromosome preps. Initially the culture was going fine. We seeded it to keep a back-up and tried harvesting what we had with no success at all. All our cells lysed (we've had trou...