Search found 5 matches
Search found 5 matches • Page 1 of 1
- Fri Mar 30, 2012 11:32 pm
- Forum: Cell Biology
- Topic: Subcellular Fractionation question
- Replies: 1
- Views: 2396
You're going to get 4 fractions when you're doing this Cytosolic proteins Membrane proteins Nuclear Proteins Cytoskeletal proteins For example in the Qproteome kit it breaks open the plasma membrane but doesn't solubilise it you spin that down the supernatant are the cytosolic proteins but once you'...
- Sat Sep 25, 2010 1:43 pm
- Forum: Molecular Biology
- Topic: How to determine success of a digest producing short segment
- Replies: 4
- Views: 3339
I would say it most likely did run off on a 1.5% gel, if it were me trying that I would try a 5% agarose gel also @jackbean for big segments of dna it is best to use a low % gel instead of a high one.
So I did a plasmid mini prep and you forgot to use RNAse would it be suitable for cloning, i'm guessing not but hoping yes. I will be running a gel tomorrow to check it and i'm pretty sure i'll need to repeat it Right now it's in -20 dissolved in distilled water or something could I possibly centrif...