Search found 6 matches

by pmngrad
Wed Sep 01, 2010 4:15 pm
Forum: Genetics
Topic: Poly(A) Signal
Replies: 2
Views: 2074

Re: Poly(A) Signal

Both genes would be in frame - unsure about the exact Poly(a) signal though. I would assume that it would be a AAUAAA or equivalent sequence.

Thanks for your help! :)
by pmngrad
Tue Aug 31, 2010 11:49 pm
Forum: Genetics
Topic: Poly(A) Signal
Replies: 2
Views: 2074

Poly(A) Signal

When a poly(a) signal is placed downstream of a gene sequence wouldn't this cause the termination of transcription before the gene of interest is actually expressed?
by pmngrad
Sun May 16, 2010 7:06 pm
Forum: Molecular Biology
Topic: Dissociation Constants
Replies: 1
Views: 1653

Dissociation Constants

Quick question of clarification, If a particular receptor has a dissociation constant (Kd) of 50nM for instance. That means that the receptor is half bound by ligand when the concentration of the ligand is 50nM. To fully saturate a receptor, would one use twice as much ligand? 100nM in this case? Th...
by pmngrad
Tue May 04, 2010 8:59 pm
Forum: Cell Biology
Topic: Cell Wash Out
Replies: 1
Views: 3043

Cell Wash Out

In cell culture publications, I have seen some protocols where following a specific treatment, cells are ' washed out ' and 'equilibriated' prior to lysis and collection for biochemical examination. I have also seen protocols using the same treatments where the authors did not ' wash out ' cells, an...
by pmngrad
Fri Apr 23, 2010 2:25 pm
Forum: Genetics
Topic: eGFP Expression Efficiency
Replies: 3
Views: 1822

Re: eGFP Expression Efficiency

Thanks for that response - I'll look into what silencing can do to play into this.

I think that the actual terms I should have used for this question is:

What would cause reduced eGFP fidelity in some cells and not others. I'm not sure if that key word triggers anyone's brains! Thanks again!
by pmngrad
Thu Apr 22, 2010 5:30 pm
Forum: Genetics
Topic: eGFP Expression Efficiency
Replies: 3
Views: 1822

eGFP Expression Efficiency

Hi All, Say you have a transgenic mouse who has been genetically modified to express eGFP where ever Protein X is expressed. What events might cause eGFP not to be expressed in a cell, when you are certain that Protein X is expressed. For example, say you are looking at two cells fluorescently stain...