Search found 4 matches

by crama
Thu Mar 11, 2010 9:17 am
Forum: Molecular Biology
Topic: Problem with micro BCA assay
Replies: 1
Views: 1297

Problem with micro BCA assay

hi,

I am using micro BCA protein Assey kit, when i am using fresh D.water it gives correct assay but when i am using old D.Water , with out add of any sample (protein), color change.
suggest me why it is happening
by crama
Thu Mar 11, 2010 9:05 am
Forum: Molecular Biology
Topic: SDS-PAGE migration conditions
Replies: 4
Views: 3703

when you reduce gel % , it migrates much faster than higher %, but there is no chances to burn gel at all. you can choose v constant or amp constant.

rama
by crama
Thu Mar 11, 2010 8:56 am
Forum: Molecular Biology
Topic: When is sterilization necessary?
Replies: 1
Views: 1292

Re: When is sterilization necessary?

There is no need for biohazard hood, this test can be done, out site in lab. but make sure that your regents are fresh and prepared in the wfi, if you filter it with 0.2 u filter it better

Rama
by crama
Mon Feb 22, 2010 2:07 am
Forum: Bioinformatics
Topic: Biotechnology forum
Replies: 13
Views: 27984

Re: Biotechnology forum

Please visit http://www.biofriend.info good forum