Search found 2 matches

by Zabulius
Tue Feb 02, 2010 4:20 am
Forum: Molecular Biology
Topic: Biology Lab Help - Protein Assays
Replies: 4
Views: 9220

Re: Biology Lab Help - Protein Assays

Yes, atleast I believe so. It involves the dye Coomassie Blue. The dye binds to both basic and aromatic amino acids when reacted with a protein sample. I also know the absorbance of the dye shifts when mixed with a protein sample but other than that I can't say much.
by Zabulius
Mon Feb 01, 2010 10:08 pm
Forum: Molecular Biology
Topic: Biology Lab Help - Protein Assays
Replies: 4
Views: 9220

Biology Lab Help - Protein Assays

Finished all parts of my lab and report except this final question. I'm not quite sure I fully understand the concept of why it happens. Most proteins have an absorption maximum at 280 nm. If that is so, why do you assess the absorbance in the Bradford assay with the wavelength set at 595 nm? (2 rea...