Search found 18 matches

by Bill123
Thu Mar 18, 2010 12:31 am
Forum: Molecular Biology
Topic: Double digestion
Replies: 2
Views: 1256

Re:

JackBean wrote:I don't know. Try to write it as here.



:P Thank you, Jack!!
by Bill123
Tue Mar 16, 2010 9:24 pm
Forum: Molecular Biology
Topic: Double digestion
Replies: 2
Views: 1256

Double digestion

Hello everybody, I have constructed a gene library, which is made from two parental genes (gene 1 and gene 2). Now I want to say I have digested this library with two restriction enzymes, so, is this sentense correct: "The two restriction enzymes are selected corresponding to the single restric...
by Bill123
Fri Feb 19, 2010 4:56 pm
Forum: Molecular Biology
Topic: quality of biological Paper
Replies: 2
Views: 1795

Re:

JackBean wrote:if the paper presented brand new things, than you should put x behind 3



Thank you very much !!!! :mrgreen: :D :)
by Bill123
Thu Feb 18, 2010 11:24 pm
Forum: Molecular Biology
Topic: quality of biological Paper
Replies: 2
Views: 1795

quality of biological Paper

I received a question list to ask the quality of a paper. I do not understand what is the meaning of "Succeeds by a large amount"? It means the paper meet the criterion very well? For example, if I want to say that the information presented in the paper is very new, I should place an x aft...
by Bill123
Mon Dec 21, 2009 8:53 pm
Forum: Molecular Biology
Topic: about E. coli
Replies: 4
Views: 1839

Re:

:D :mrgreen: Thank you, I will follow your suggestion.

JackBean wrote:If you leave the 'ninty-six' in the beginning, I think, it could be quite fine ;)
by Bill123
Mon Dec 21, 2009 12:21 am
Forum: Molecular Biology
Topic: about E. coli
Replies: 4
Views: 1839

Re:

:P :D So you means the sentence is correct??


JackBean wrote:you don't have to start new topic everytime :roll:

I think, it's quite clear, you've picked up 96 colonies, if you used 96-well plate ;)
by Bill123
Sun Dec 20, 2009 10:37 pm
Forum: Molecular Biology
Topic: about E. coli
Replies: 4
Views: 1839

about E. coli

Is this sentence correct? I want to discribe that I want pick up single colonies and culture them in 96-well PCR plate. Thank you!!! :( :) :D

Ninety-six colonies were carefully picked up, and suspended in 30 µl of media in the wells of a 96-well PCR plate.
by Bill123
Sun Dec 20, 2009 9:52 pm
Forum: General Discussion
Topic: about e. coli colonies
Replies: 2
Views: 1313

Re:

No special context, I just want to show that my colony picking machine is efficient. So the sentence is correct or not??


mith wrote:context?
by Bill123
Sun Dec 20, 2009 10:35 am
Forum: General Discussion
Topic: about e. coli colonies
Replies: 2
Views: 1313

about e. coli colonies

I am writting a thesis, can you please tell me this sentense is correct or not?? I just want to show that my colony picking machine is efficient. Thank you. :D :P :?: :mrgreen:

"all the colonies (4×96=384) were conveniently picked up in a single day"
by Bill123
Sat Nov 28, 2009 4:25 pm
Forum: Genetics
Topic: QuikChange
Replies: 2
Views: 1219

Re:

Yes, I am just discribing the site directed mutagenesis. :D


JackBean wrote:available?

What about 'appear' instead?
BTW isn't that quite clear, that it will be there after directed mutagenesis?
by Bill123
Fri Nov 27, 2009 8:49 pm
Forum: Genetics
Topic: QuikChange
Replies: 2
Views: 1219

QuikChange

Hello everyone: I am writing a thesis, I have a scentence which is very difficult for me. Would you please tell me is it very correct?? Thank you very much. "However, a clear main DNA band in agarose gel corresponding to the expected size of the recombinant plasmids (~5kb) was available from th...
by Bill123
Mon Nov 23, 2009 9:11 pm
Forum: Microbiology
Topic: is this correct????
Replies: 3
Views: 1608

Re:

JackBean wrote:Except, that both your primers seem tzo have the same sequence, it looks fine to me.



:D Thank you for your help!!