Search found 3 matches

by denrul
Sun Oct 25, 2009 3:34 pm
Forum: Molecular Biology
Topic: cloning and activity problem
Replies: 5
Views: 2986

inserts long 2 kb, 2.5 kb and 4,5 kb respectivelly.
by denrul
Sun Oct 25, 2009 2:16 pm
Forum: Molecular Biology
Topic: cloning and activity problem
Replies: 5
Views: 2986

1) Yes I have sequenced my fragment and of course I selected my clone via white/blue screen. 2) When inducing with IPTG, Is quantity of mRNA increase?? Basically; increasing mRNA quantity cause increasing protein quantity so increasing activity of protein. I want to ask you is ribosome use SD sequen...
by denrul
Sun Oct 25, 2009 1:22 pm
Forum: Molecular Biology
Topic: cloning and activity problem
Replies: 5
Views: 2986

cloning and activity problem

Hi there; I have two question about my cloning attempt, 1) I have cloned three different genes (originated from bacillus) in to LacZ of pSK(-) (pbluescript) vector. After transformation E.coli DH5alpha and induce with 0.5 mM IPTG there is no difference on SDS gel between Host and Clones. Cloned gene...