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by MattNYC
Mon Oct 19, 2009 7:17 pm
Forum: Molecular Biology
Topic: Spin-down speed for HEK293 after aantibody binding?
Replies: 2
Views: 6353

Spin-down speed for HEK293 after aantibody binding?

Hello, I do antibody binding test with anti-human-IgG-PE and for the other assay with biotinylated antibody and streptavidin-apc-cy7 as the second AB. I am using 293T cells for the assay. Since the pellett is very loose I would like to increase the centrifugation speed during the washing steps. Now ...