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by Nodo
Mon Oct 12, 2009 3:16 pm
Forum: Molecular Biology
Topic: BAC linearization
Replies: 0
Views: 746

BAC linearization

Hi, I just finished my BAC modification to create a new transgenic mouse line. Now I have to purify this construct to inject it to zygotes. I know that it's better to linearize the BAC before injection however, I wondered if it is a problem if the injected construct still contain the antibiotic resi...