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by shuk08
Tue Oct 06, 2009 12:29 pm
Forum: Molecular Biology
Topic: nested pcr
Replies: 3
Views: 2321

nested pcr

How to get rid of larger non-specific bands appearing in 1st round of pcr just above the expected bands (of approx. 1.5 kb)? The non-specific bands from the 1st pcr are amplified in 2nd round of pcr using nested pair of primer.