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- Tue Sep 08, 2009 11:31 am
- Forum: Molecular Biology
- Topic: Problems with gene knockdown
- Replies: 1
- Views: 4380
We use RNAi and Lentiviral transgenesis in our lab to generate transgenic mice. We use luceferase assay to validate the shRNA sequences. The problem is the results don't seem to traslate well into in vitro systems. I analysed 8 sequences with the assay and made virus with them. The virus / plasmid h...