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by zodiac1505
Tue Sep 08, 2009 11:31 am
Forum: Molecular Biology
Topic: Problems with gene knockdown
Replies: 1
Views: 4380

Problems with gene knockdown

We use RNAi and Lentiviral transgenesis in our lab to generate transgenic mice. We use luceferase assay to validate the shRNA sequences. The problem is the results don't seem to traslate well into in vitro systems. I analysed 8 sequences with the assay and made virus with them. The virus / plasmid h...