Search found 4 matches

by itamo
Sun Feb 17, 2013 5:21 am
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 4958

I cooked it on a hot plate on low temp with a stirring magnet and in the end put it in a microwave oven on low-medium for a couple of soconds a few times to get rid of the bubbles. and after getting a clear solution i added the atidium bromide.
worked great.
by itamo
Tue Feb 12, 2013 6:43 am
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 4958

How to make high concentration agarose gel

Thanks.

Do you cover the flask to avoid evaporation ?
If you do, with what ?
Did you tried it yourself?
How many cycles of heating and swirling did you do?
by itamo
Mon Feb 11, 2013 1:32 pm
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 4958

How to prepare a 3.5% agarose gel ?

I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, but there were alot of bubbles in the gel.

Is there a way to prepare a high concentration gel without the annoying bubbles?
by itamo
Mon Aug 24, 2009 4:20 am
Forum: General Discussion
Topic: Injection of pellets
Replies: 0
Views: 2135

Injection of pellets

I have to inject a treatment pellets to test animals in the neck, as described here:
http://www.protocol-online.org/forums/i ... topic=9090

Does someone have a tested method on how to do it ?