Search found 3 matches

by apolll
Sun Aug 23, 2009 8:55 pm
Forum: Cell Biology
Topic: b cells and active immunity
Replies: 2
Views: 5339

I don't exactly know an answer to this. It's just that I remember that there isn't a decision in cell fate that early. Meaning first all naive B cells become plasma cells. Only after the inflammation most of the plasma cells die but some of the plasma cells become memory cells. At least that's what ...
by apolll
Sun Aug 23, 2009 8:40 pm
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 11099

Re: PCR & Agarose gel electrophoresis

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. I'd e...
by apolll
Sun Aug 23, 2009 8:23 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 33842

separation problem with GelRed

Hello! We recently switched from ethidium bromide to Gelred in our lab. (We always prestain gels - poststaining is no option because its too inconvenient and timeconsuming - using 1xTAE buffer). Our DNA samples and the ladders aren't separated as sharply as it was when we used ethidium bromide. I al...