Search found 3 matches

by claudiupopa
Sun May 03, 2009 9:42 am
Forum: Molecular Biology
Topic: Calculating DNA concentration in PCR product
Replies: 6
Views: 19941

Re: Calculating DNA concentration in PCR product

yes but the spec can be set off by other components in the pcr mix, like leftover primers and nucleotides. Any way to circumvent that? i need accurate readings of the concentration
by claudiupopa
Sat May 02, 2009 3:48 pm
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 8
Views: 33371

Re: Prep. of 50X TAE buffer

Unfortunately im not familiar with making TAE but i can give you some general advice. In this case you have to take in consideration not only the pH of the PBS solution but also the effect of the acetic acid (which will be "pulling" your pH down into acidic) so if yout pH is to low try low...
by claudiupopa
Sat May 02, 2009 3:18 pm
Forum: Molecular Biology
Topic: Calculating DNA concentration in PCR product
Replies: 6
Views: 19941

Calculating DNA concentration in PCR product

I need to figure the concentration of amplified DNA after a PCR by using available information like concentration of template DNA, enzyme speed, number of PCR cycles and so forth. Someone told me that i should be able to figure it out myself but im really bad with numbers and math so i got nowhere. ...