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by grdnfs20
Thu Apr 30, 2009 10:25 pm
Forum: Molecular Biology
Topic: Lymphocyte Overnight Culture parameters
Replies: 5
Views: 10561

Re: Lymphocyte Overnight Culture parameters

Im collecting human blood samples and isolating lymphocytes using Ficoll-Hypaque method. I am trying to culture the cells and treat them to various oxidation reactions. I am having trouble with the cell culture, there does not seem to be any control over the cell cultures, for instance i plate 500,0...