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- Thu Apr 30, 2009 10:25 pm
- Forum: Molecular Biology
- Topic: Lymphocyte Overnight Culture parameters
- Replies: 5
- Views: 10561
Re: Lymphocyte Overnight Culture parameters
Im collecting human blood samples and isolating lymphocytes using Ficoll-Hypaque method. I am trying to culture the cells and treat them to various oxidation reactions. I am having trouble with the cell culture, there does not seem to be any control over the cell cultures, for instance i plate 500,0...