Search found 8 matches

by SRR
Thu Nov 29, 2018 12:00 pm
Forum: Microbiology
Topic: Can bacteria grow on MES as a carbon source?
Replies: 3
Views: 7250

Re: Can bacteria grow on MES as a carbon source?

I think I can now answer my own question: While some Good's buffers are subject to degradation by bacteria, MES isn't one of them. It seems the reason for the difference is that MES has lower metal binding constants than tricine. I can achieve a similar bacteria bloom effect by keeping the concentra...
by SRR
Wed Nov 28, 2018 12:54 pm
Forum: Microbiology
Topic: Which flagellate alga is easiest to culture?
Replies: 0
Views: 3555

Which flagellate alga is easiest to culture?

Hi All,

So ... can anyone tell me which species or genus of freshwater flagellate eukaryotic algae would be easiest to grow in liquid culture?
No hay infusion though please, I would prefer a more defined medium.

Thanks in advance,

Shaun.
by SRR
Tue Apr 03, 2018 2:22 pm
Forum: Microbiology
Topic: Can bacteria grow on MES as a carbon source?
Replies: 3
Views: 7250

Can bacteria grow on MES as a carbon source?

Hi All, I have recently made up AF6 culture medium with MES (2-Morpholinoethanesulfonic Acid). The culture became slightly turbid overnight. I have previosly tried a very similar medium with tricine instead of MES, but that didn't become turbid overnight. I have done other experiments where I added ...
by SRR
Thu Jan 18, 2018 2:17 pm
Forum: Microbiology
Topic: Why does Pfennig's medium contain potassium chloride?
Replies: 0
Views: 7439

Why does Pfennig's medium contain potassium chloride?

Pfennig's medium contains ammonium chloride, potassium phosphate and potassium chloride amongst other things. I have seen other culture media which also contain these things and there is a note on one which says that "potassium chloride provides essential ions". Why are these additional ions needed ...
by SRR
Sun Sep 17, 2017 3:36 pm
Forum: Microbiology
Topic: Why doesn't cow manure promote the growth of water mould?
Replies: 1
Views: 5221

Why doesn't cow manure promote the growth of water mould?

I have noticed that when cow manure is added to a pond, the water goes green or green/brown suggesting the growth of organisms such as cyanobacteria, chrysophytes, cryptophytes and euglenoids, it doesn't look like water mould. I find this strange becuase if I add the ingredients of cow manure (ammon...
by SRR
Mon Jul 17, 2017 7:45 pm
Forum: Microbiology
Topic: Clear in the morning, brown in the afternoon
Replies: 0
Views: 2080

Clear in the morning, brown in the afternoon

If I have some water collected from a freshwater source which is clear in the morning, but goes brown later in they day (then repeats the following day), what organisms would cause that? I have read that diatoms can produce this effect, because they divide in unison at night, but are there any other...
by SRR
Mon Jul 17, 2017 7:35 pm
Forum: Microbiology
Topic: Trouble growing Microcystis
Replies: 1
Views: 2596

Re: Trouble growing Microcystis

I found some guidelines by Christopher Bolch on researchgate which solved my problem. My mistake was mainly pH. I was trying to culture an organism from an acidic habitat under alkaline conditions. That was because I had read that if water is green and has white froth on it, then it is a cyanobacter...
by SRR
Tue Apr 18, 2017 12:21 pm
Forum: Microbiology
Topic: Trouble growing Microcystis
Replies: 1
Views: 2596

Trouble growing Microcystis

Hello all, I've been trying to grow the cyanobacterium Microcystis in liquid culture for years. I once found water in a stream in the autumn which had a really strong green/blue/brown tint, in fact a bottle of it looked almost black in certain lighting. There was also a lot of white froth on the wat...