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by ppm58
Thu Mar 17, 2016 4:45 pm
Forum: Molecular Biology
Topic: RNA recovery from cell cultured in agarose matrix
Replies: 1
Views: 3296

RNA recovery from cell cultured in agarose matrix

Hello I culture cells as 3D model (spheroid) embedded in agarose matrix. I am trying to recovery RNA from these culture to perform RT PCR but I meet several problem as RNA quantity (<30 ng/mL) and as RNA quality (260/280/230 ratio are bad). Have some ideas to obtain a fair RNA quality and quantity i...