Search found 5 matches

by protoemics
Fri Mar 20, 2015 2:19 am
Forum: General Discussion
Topic: RNA isolation from brain sections
Replies: 0
Views: 3017

RNA isolation from brain sections

I'm looking to isolate RNA from brain sections under two conditions. First are punches from fresh vibratome slices. I've been told that the time it takes to cut the sections and then punch them is too long for good RNA isolation. Is this true? Secondly, I'd like to take fresh frozen brains that are ...
by protoemics
Wed Mar 11, 2015 5:55 am
Forum: General Discussion
Topic: anti-hsp70 antibody
Replies: 1
Views: 5221

I am not sure you tried the stressgen catalog but I'm using one of their monoclonal Ab against Hsp70 on Rainbow trout and it definitely detects inducible hsp70 even though Rbw trout is not mentioned on their catalog, I did not come across one against drosophila Hsp 70 but you may want to contact the...
by protoemics
Thu Feb 05, 2015 8:23 am
Forum: Cell Biology
Topic: Do hESC actively produce telomerase?
Replies: 1
Views: 5850

Hi, I have searched some materials for you; those are about hESC; Hope they can help you !
http://www.sciencepub.net/stem/stem0303 ... 37_101.pdf
https://www.google.com.hk/url?sa=t&rct= ... wzFIcWHYmw
by protoemics
Thu Feb 05, 2015 7:56 am
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 2
Views: 12653

Questions about DNA dialysis

Dear all, I would like to use dialysis to exchange ions in my DNA. For this I bought Slide-A-Lyzer mini pots for the dialysis. First, I am looking for more information about the theoretical background of dialysis. Can you give me some literature hints, please? Secondly, I know my target DNA concentr...
by protoemics
Wed Feb 04, 2015 8:11 am
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 0
Views: 10193

Hi: Although they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqma...