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by spin2
Mon Feb 02, 2009 2:01 pm
Forum: Molecular Biology
Topic: checking quality and quanitfying cDNA
Replies: 1
Views: 2533

checking quality and quanitfying cDNA

I have been having lots of troubles gettign robust PCR reactions to work from cDNAs that I have stored in the -20. I have checked the RNA quality by gel electrophoresis and get nice rRNA bands and no degredation. However, depending on the day some of my cDNA give me products and some do not. Is ther...