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- Thu Jan 29, 2009 12:34 am
- Forum: Molecular Biology
- Topic: experiences with ni-affinity protein purification??
- Replies: 4
- Views: 13786
Your protein is inactive due to the high concentration of imidazole. Try to use a cobalt based resin and that should do the trick. You will extract and wash in absence of imidazole and then purify in low concentration s of imidazole.