Search found 4 matches

by pcrboy
Thu Jan 29, 2009 12:11 pm
Forum: Molecular Biology
Topic: G-T Mismatch Repair
Replies: 4
Views: 4427

specific enzymes, probably something similar to glycosolases
by pcrboy
Thu Jan 29, 2009 12:10 pm
Forum: Molecular Biology
Topic: Amount of DNA for eficient transformation
Replies: 4
Views: 3970

Re: Amount of DNA for eficient transformation

not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.
by pcrboy
Thu Jan 29, 2009 3:30 am
Forum: Molecular Biology
Topic: Amount of DNA for eficient transformation
Replies: 4
Views: 3970

you mean transfection? or cancer transformation?
by pcrboy
Mon Jan 26, 2009 8:17 am
Forum: Molecular Biology
Topic: Colony PCR
Replies: 2
Views: 2897

Re: Colony PCR

determining where the smear appears may help determine whether it is chromosome DNA or plasmid DNA.