Search found 16 matches

by beautifulmind420
Thu Dec 11, 2008 5:42 pm
Forum: Microbiology
Topic: DNase + or - ?
Replies: 2
Views: 6444

Pseudomonas aeruginosa - negative
Reference:
http://www.pubmedcentral.nih.gov/articl ... tid=535267

Pseudomonas fluorescens - negative
Reference
http://aem.asm.org/cgi/reprint/18/3/355.pdf (check Table 1)

Proteus mirabilis - may be positive

Citrobacter freundii - may be negative
Reference
http://jcm.asm.org/cgi/reprint/4/5/453.pdf
by beautifulmind420
Thu Dec 11, 2008 4:58 pm
Forum: General Discussion
Topic: searching for a cell culture book
Replies: 2
Views: 2014

Hey, If I were you, I would search on google. Google Book has cataloged almost everybook online and some of 'em have limited preview, so you can look around and find yourself a book of your interest. Just type your keyword and you will get lots of option. Once you know the names of books, then you c...
by beautifulmind420
Thu Dec 11, 2008 4:54 pm
Forum: Molecular Biology
Topic: Which biocemist should I do a research report on?
Replies: 5
Views: 3024

Don't forget to check out nobel speech and biography from nobelprize.org
by beautifulmind420
Tue Dec 09, 2008 9:39 pm
Forum: General Discussion
Topic: SDS-PAGE and specific activity
Replies: 1
Views: 1812

Hi, I am not sure if this is the right explanation, but here is something to think about... At each purificiation step, the number of bands should be decreased. In theory, the protein should be pure when subsequential purification do not lead to any increase in specific activity. So as the number of...
by beautifulmind420
Tue Dec 09, 2008 9:35 pm
Forum: Genetics
Topic: When are parts of prokaryotic operons seperated?
Replies: 5
Views: 7002

Textbook: iGenetics by Peter J. Russell
by beautifulmind420
Tue Dec 09, 2008 7:57 pm
Forum: Molecular Biology
Topic: Help please! Directional cloning: colonies with WEIRD DNA?
Replies: 1
Views: 2698

Hi,

Just a question: Did you send your sample for sequencing to check if there is any mutation in gene of interest after transformation?
by beautifulmind420
Tue Dec 09, 2008 7:53 pm
Forum: Molecular Biology
Topic: What are some good books?
Replies: 4
Views: 2237

Above mentioned books are really good. I think everyone who wants to pursue their career in research, should have these books. You can also try to find on google book (just write the key words!). Some of them have limited preview, so it will give you more options. I hope this helps!
by beautifulmind420
Tue Dec 09, 2008 7:47 pm
Forum: Molecular Biology
Topic: Gene cloning
Replies: 3
Views: 3046

Use gateway cloning, it's relatively fast. Inhibiting regulatory domain of enzyme can help you in changing catalytic activity (i.e. nonsense mutation may give you gain-of-function)
by beautifulmind420
Tue Dec 09, 2008 7:42 pm
Forum: Molecular Biology
Topic: DNA hybridization
Replies: 4
Views: 3233

It is unwise because there are multiple codons exist for one amino acid!
by beautifulmind420
Tue Dec 09, 2008 7:39 pm
Forum: Microbiology
Topic: Help Needed Please
Replies: 2
Views: 1901

These are basic concepts, you should be able to find online for sure.
by beautifulmind420
Tue Dec 09, 2008 7:33 pm
Forum: Genetics
Topic: can a school boy do some genetic experiment?
Replies: 4
Views: 3547

Be a Gregor Mendel! Do some pea experiments by yourself.
by beautifulmind420
Tue Dec 09, 2008 7:28 pm
Forum: Genetics
Topic: When are parts of prokaryotic operons seperated?
Replies: 5
Views: 7002

Form my understanding, polycistronic sites means 1 mRNA is responsible for production of more than one proteins. I know from one experiment that if you have nonsense mutation in lacZ gene, it would result in truncation in lacZ protein and lacY and lacA proteins wouldn't be produce at all.