Search found 9 matches

by Jamus
Mon May 11, 2009 9:14 am
Forum: Genetics
Topic: What is meant by "single-step mutation"?
Replies: 1
Views: 6038

What is meant by "single-step mutation"?

Googling the term above did not help. Can anyone possibly explain the concept to me? Thank you

Jamus
by Jamus
Sat Apr 25, 2009 12:49 pm
Forum: Cell Biology
Topic: Inhibition of enzyme - will it result in upregulation?
Replies: 0
Views: 2705

Inhibition of enzyme - will it result in upregulation?

Hey all, I am trying to figure out a mystery here. In an experiment that I've done a few weeks ago, ketoconazole (CYP inhibitor) seems to result in an overall increase in the quantity of the CYP 450 proteins in the liver. I can understand how this works if it is a CYP inducer, but... can an inhibito...
by Jamus
Wed Jan 07, 2009 4:34 am
Forum: Molecular Biology
Topic: Confusion over terms related to genetics
Replies: 2
Views: 2330

Confusion over terms related to genetics

Greetings all, I’m experiencing slight confusion over the difference and meaning of these terms: 1. Mutation 2. Polymorphism 3. Variants On top of these, how do we know when to use the prefix “disease associated…” with the above terms? For instance, “disease associated polymorphism”. Besides this, I...
by Jamus
Wed Dec 10, 2008 3:47 am
Forum: Bioinformatics
Topic: NMR
Replies: 2
Views: 2863

I've always like ChemGuide when it come to introduction to basics.

Here's the link for NMR: http://www.chemguide.co.uk/analysis/nmrmenu.html

Good luck :)
by Jamus
Wed Dec 10, 2008 3:42 am
Forum: Molecular Biology
Topic: How to read tricky electropherograms?
Replies: 8
Views: 8474

Let me guess!

On the forward strand, the forward primer will sequence both the top DNA chain of the "Mom" and "Dad", and that's why you get "A" and "G" peaks in the electropherogram for the forward strand?
by Jamus
Wed Dec 10, 2008 3:30 am
Forum: Molecular Biology
Topic: How to read tricky electropherograms?
Replies: 8
Views: 8474

Re: How to read tricky electropherograms?

Thanks for the prompt reply In the diagram attached, on one particular gene, the alleles from the parents of a person is different at that particular point. Which bases are detected in the electropherogram? If you are finding my questions too "frustratingly" to answer, could you please ref...
by Jamus
Tue Dec 09, 2008 8:14 pm
Forum: Molecular Biology
Topic: How to read tricky electropherograms?
Replies: 8
Views: 8474

Re: How to read tricky electropherograms?

Yeah, I am doing a PCR genomic DNA from a diploid organism i.e. Human.

I think I require some basic explanations in the first place. How can polymorphism arise in such a way that TWO nucleotides occupy the same spot?

Thanks.
by Jamus
Tue Dec 09, 2008 8:59 am
Forum: Molecular Biology
Topic: How to read tricky electropherograms?
Replies: 8
Views: 8474

Re: How to read tricky electropherograms?

Thanks for the reply. How can a nucleotide site be heterozygote (i.e. have two bases at the same time)?
by Jamus
Mon Dec 08, 2008 8:08 am
Forum: Molecular Biology
Topic: How to read tricky electropherograms?
Replies: 8
Views: 8474

How to read tricky electropherograms?

Dear all, (Please see attached file). I am having complications with reading electropherograms. In the attached file, how do you decide on what base it is and what makes you think so? What does it mean by "heterozygotes" in the context of electropherograms? How does that arise? Thank you. ...