Search found 2 matches

by watsond
Wed Dec 03, 2008 11:15 am
Forum: Molecular Biology
Topic: Lymphocyte Overnight Culture parameters
Replies: 5
Views: 10646

Re: Lymphocyte Overnight Culture parameters

After trying out various culture parameters, I found that the following worked best.

Cell washing medium (for centrifuge): RPMI + 2% FCS
Cell culture medium : RPMI + 15% FCS
5% CO2 _at_ 37C.

After 24h incubation, necrotic cells were around 0.6% and late apoptotic cells 4.5%
by watsond
Thu Nov 27, 2008 12:31 am
Forum: Molecular Biology
Topic: Lymphocyte Overnight Culture parameters
Replies: 5
Views: 10646

Lymphocyte Overnight Culture parameters

I am doing some work with human lymphocytes (or rather PBMCs) with regard to DNA repair of oxidative damage after overnight incubation. The only problem is that the overnight incubation causes varying amounts of dead or dying cells, as detected by using the annexin V and PI stains with a FACS cytome...