Search found 2 matches

by TurdFerguson
Fri Oct 31, 2008 10:46 pm
Forum: Molecular Biology
Topic: Too much DTT
Replies: 0
Views: 1498

Too much DTT

I set up a reaction recently in which I was examining a cytosolic chaperone's activity. It is possible I may have had 10 mM DTT in the reaction buffer, rather than the suggested 1 mM DTT. Is it possible this could interfere with my protein's activity?

-Undergrad dude
by TurdFerguson
Tue Oct 28, 2008 3:23 am
Forum: Cell Biology
Topic: Mitochondrial Fission/Fusion and the UPS
Replies: 0
Views: 870

Mitochondrial Fission/Fusion and the UPS

Quick question. How is mitochondrial fission and fusion dependent on the UPS? I'm reading a paper right now that speculates lower proteasomal activity might affect mitochondrial activity via this mechanism. I was wondering how, since it doesn't give specifics.