Search found 4 matches

by khushi
Thu Oct 30, 2008 3:12 am
Forum: Molecular Biology
Topic: Interesting question....
Replies: 1
Views: 1774

Interesting question....

A bizzare organism was isolated from deep space probe and found its cellular components very similar to terrestrial organism.when the organism was examined using the meselson stahl experiment only bands of heavy DNA were isolated in repetitive cell divisions.The organism appeared to have all the typ...
by khushi
Sat Oct 25, 2008 3:03 am
Forum: Molecular Biology
Topic: Restricion map.
Replies: 3
Views: 2221

Re: Restricion map.

Yeah if it would have been same length then it would have been easy to make map....that's what is confusing me. Isolated gene from an EcoR I digest contains a single Asp 1 restriction site but no other restriction sites.The gene is inserted into a plasmid digested with Ecor 1 and the plasmid with th...
by khushi
Fri Oct 24, 2008 3:04 pm
Forum: Molecular Biology
Topic: Molecular weight of protein
Replies: 1
Views: 2532

Molecular weight of protein

This is my assignment. A pure protein was subjected to N-terminal analysis using c14-phenyl isothiocyanate,specific activity 120ci/mole.Reaction of the reagent with 2.3microgram of protein produced 12,300 cpm PTH-met and 8200 cpm PTH-val with a 90% counting efficiency.Calculate molecular weight of p...
by khushi
Fri Oct 24, 2008 2:45 pm
Forum: Molecular Biology
Topic: Restricion map.
Replies: 3
Views: 2221

Restricion map.

I am trying to construct restriction map with following data..

E cor1--------2

Ecor 1+Asp 1.....1.6,.4

Asp 1....0.9,2.1,2.4,3.6.

I am trying to make linear map.Can anyone help?