Search found 11 matches

by domwood
Tue Apr 12, 2011 3:13 pm
Forum: Molecular Biology
Topic: How to joint two cloning genes?
Replies: 3
Views: 2977

Re: How to joint two cloning genes?

Blunt Ended Ligation?
by domwood
Mon Apr 05, 2010 7:07 pm
Forum: Molecular Biology
Topic: Biochemistry help
Replies: 2
Views: 1716

Re: Biochemistry help

I don't know anything about your specific enzyme, and I may be mis-understanding your project, but I guess from a Molecular Biology standpoint the route I would follow would be: 1) Select the sequences you want included to make your enzyme, the metalloprotease sequence and ColIII sequence. 2) Design...
by domwood
Mon Apr 05, 2010 6:46 pm
Forum: Molecular Biology
Topic: RNA prep help
Replies: 4
Views: 4529

Re: RNA prep help

I use RNAeasy Kits all the time and I find that the key factors are the quality of RNA and the control of contamination. Whatever you do to remove RNAses there will always be some present so I try to minimize the time between extracting RNA from it's native source and using it. If it's a tissue extr...
by domwood
Wed May 20, 2009 10:32 am
Forum: Genetics
Topic: knocking out which genes can make human live longer
Replies: 2
Views: 4296

This seems a little pointless, the genes will effect each other in ways that you can't predict. Plus where did you get those genes from? I did a NCBI search and some of them aren't even human?
by domwood
Wed May 20, 2009 10:25 am
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 3497

Well I'm no expert on this, but I would have thought the two key steps are the physical ones, the gel and the centrifugation. Have you tried running the gel at a lower voltage over a longer period, and also centrifuging for longer. Also what is the source of the DNA, can you be sure of the extractio...
by domwood
Sun Feb 15, 2009 3:11 pm
Forum: Molecular Biology
Topic: Fungal DNA extrtaction problem
Replies: 2
Views: 2928

I'm probably totally wrong here, but how much do you know about the fungal DNA. 15kb piece seems to me to be coincidentally 50% of 30kb. Is it possible the the DNA in the fungus is two separate chromosomes?
by domwood
Mon Feb 09, 2009 7:59 pm
Forum: Genetics
Topic: AB x A (Blood type) Cross Issues and Trihybrid Phenotypes
Replies: 1
Views: 9233

There's nothing wrong with your answers for the first one. As for the second one you're on the right track. Why doesn't it seem right that that none are O. Remember that the blood groups refer to surface antigens of blood cells, A group just has A antigen, B just has B antigen, AB has both, and O ha...
by domwood
Mon Feb 09, 2009 7:31 pm
Forum: Genetics
Topic: Karyotyping of Plants
Replies: 2
Views: 3177

The number of chromosomes and their ploidy are somewhat arbitrary and you will probably find it difficult to infer anything from comparing karyotypes. For example cats have 38 chromosomes, Humans 46, Dogs 78, and they are all mammals, trying to infer any 'relationship' would be difficult. It's not s...
by domwood
Sun Dec 14, 2008 5:55 pm
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 13113

Re: dideoxy chain-termination method for sequencing DNA

To answer each point.. 1. The distance between each nucleotide is smaller than any of our current mangnification equipment can resolve. You would not be able to see the different colors. Although there has been recent research into putting DNA sequences individually into nanotubes and measuring the ...
by domwood
Thu Oct 23, 2008 3:14 pm
Forum: Genetics
Topic: Incomplete dominance, sex-linked and Dihybrid crosses
Replies: 4
Views: 7220

This would be the sex-linked part of the title, colour blindnesses is an X linked disease. The cross is X"Y with X"X (X' is the X chromosome with the colour blind allele) So you just need to get do a crossing table to work out the four possible genotypes. Also When working out the phenotyp...
by domwood
Thu Oct 23, 2008 3:01 pm
Forum: Genetics
Topic: can a school boy do some genetic experiment?
Replies: 4
Views: 3547

Re: can a school boy do some genetic experiment?

I have one relatively easy suggestion. You could do some simple observational tests to find out some gene alleles (variations of genes) you and your family share. Look up human mendelian traits that are physical features (like cleft chins, earlobe attachment, etc.), and see what features you do and ...