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by RompingRibozyme
Wed Oct 22, 2008 9:03 pm
Forum: Molecular Biology
Topic: single stranded cDNA storage
Replies: 6
Views: 22543

Re: single stranded cDNA storage

Dear Susi, The RNA you extract is single stranded with the exception of small RNA which have self complimented and have been cleaved as we see with RNAi. When you perform an RT-PCR, you are using the ssRNA as a template and your resulting amplicon is a ssDNA known as cDNA. cDNA is always single stra...