Search found 5 matches

by faerstd
Sun Nov 30, 2008 3:57 pm
Forum: General Discussion
Topic: SDS-PAGE and specific activity
Replies: 1
Views: 1869

SDS-PAGE and specific activity

Hello, I purified an enzyme and took samples after each purification step which I loaded on SDS-PAGel and ran SDS-PAGE. I also measured the activities of each sample and calculated the specific activities of them. To my question, I've heard some time that you can somehow interprete the number of ban...
by faerstd
Thu Oct 23, 2008 2:24 pm
Forum: Molecular Biology
Topic: any problems with 1-base overhang?
Replies: 0
Views: 1048

any problems with 1-base overhang?

Hi, another question.. but this time I researched via google and didn't found anything unless i gave the wrong keywords so.. I have fragments with 1-base overhangs and want them to ligate in a vector with complementary 1-base overhangs... my teacher said that if I give these fragments in a high amou...
by faerstd
Mon Oct 20, 2008 7:43 pm
Forum: Molecular Biology
Topic: RNA precipitation after extraction: role of LiCl ?
Replies: 2
Views: 11913

Re: RNA precipitation after extraction: role of LiCl ?

i'm so stupid.. i searched google indeed, but didn't read that !!!! :lol:

so this licl specially gets large dehydrated RNA molecules make aggregates so that the precipitation works better

thanks a lot.. :oops:
by faerstd
Mon Oct 20, 2008 10:55 am
Forum: Molecular Biology
Topic: DNA hybridization
Replies: 4
Views: 3341

For the hybridization I would say the specificity (100% match with target sequence) and amount of the probes (should be in excess..)
then maybe high washing temperature and low salt concentration to wash away the unspecific hybridization..

hope it'll help..
by faerstd
Mon Oct 20, 2008 10:30 am
Forum: Molecular Biology
Topic: RNA precipitation after extraction: role of LiCl ?
Replies: 2
Views: 11913

RNA precipitation after extraction: role of LiCl ?

Hi, I have to write my protocol after a course in Molecular Biology, where in one lecture we extracted the RNA from the cells. After doing so, we precipitated the RNA with Ethanol and Lithium chloride for 3 hours. Now my question: what's the role of LiCl in this precipitation? Wouldn't the RNA be pr...