Search found 5 matches

by proteinz
Sat Aug 01, 2009 12:14 pm
Forum: Molecular Biology
Topic: northern blot analysis
Replies: 2
Views: 3230

northern blot analysis

hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help... i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp an...
by proteinz
Mon Oct 13, 2008 3:48 pm
Forum: Molecular Biology
Topic: protein function with DTT treatment
Replies: 1
Views: 3051

protein function with DTT treatment

hi all, no idea where to post this> but has any one ever heard of a protein that increases its chaperone activity following reduction with 5mM DTT?
thanks
by proteinz
Tue Oct 07, 2008 6:54 pm
Forum: Molecular Biology
Topic: beer lambert law
Replies: 3
Views: 14332

Re: beer lambert law

thanks so much guys! you've cleared it up completely.
have a nice day! :wink:
by proteinz
Tue Oct 07, 2008 4:10 pm
Forum: Molecular Biology
Topic: beer lambert law
Replies: 3
Views: 14332

beer lambert law

hi all im using the beer law to find my protein's concentration at A280. c= A/(epsilon)(c) i have found a paper which says the concentration of CS was determined using molar absorption coefficent of 1.78 for a 1mg/ml solution. WHAT DOES THIS 1MG/ML SOLUTION MEAN. IS BELOW CORRECT is this correct the...
by proteinz
Tue Oct 07, 2008 10:37 am
Forum: Molecular Biology
Topic: Citrate synthase stock calculation
Replies: 1
Views: 2055

Citrate synthase stock calculation

hi all, new to this board so unsure where to post this. i have a calculations based question, that i am having trouble with. the problem is i am trying to do a CS thermal aggregation assay on a fluorescence spectrophotometer and i cant seem to get the same light scattering units (not as high) as pre...