Search found 37 matches

by jasmina
Thu Nov 04, 2010 7:41 am
Forum: Cell Biology
Topic: western phospho-p44/42 MAPK bands
Replies: 0
Views: 1330

western phospho-p44/42 MAPK bands

Hi there! I have an questions about p-ERK bands. Previous time,after detection, usually i can got 2 P-erk bands( 44KDa and 42KDa), but this time after 1st time detection,it seems like there are a white bands in the middle of 2 bands, I detected again by chaning ECL solution, this time I only got one...
by jasmina
Thu May 20, 2010 2:37 am
Forum: Cell Biology
Topic: solvent which dissolve miconazole, in vivo experiment
Replies: 2
Views: 5062

solvent which dissolve miconazole, in vivo experiment

Hi, there I will do in vivo experiment using Miconazole, after searched information about miconazole, i found that miconazole is slight soluble in EtoH and water, although it has high soluble in DMSO, DMSO has toxic effect. My model animal is mouse, I will use 25mg/ml Miconazole solution for in vivo...
by jasmina
Tue Apr 20, 2010 10:25 am
Forum: Cell Biology
Topic: western loading sample shape
Replies: 0
Views: 846

western loading sample shape

Hi there!
Attachment is picture of my loading samples.At the begining of electrophoresis ( in 30mins, stacking gel), band shape is normal. I am not sure what happened although this did not affect detection results.
by jasmina
Wed Apr 14, 2010 5:21 am
Forum: Cell Biology
Topic: Cetylpyridinium chloride solution storage
Replies: 4
Views: 4524

thank you !^_^
by jasmina
Wed Apr 14, 2010 1:21 am
Forum: Cell Biology
Topic: Cetylpyridinium chloride solution storage
Replies: 4
Views: 4524

Re:

MrMistery wrote:if it was sterile, then it should probably be fine. Just try it and see if it works


yes, i filter it with 0.2um filter, and now it works. i want to know can i store this solution at 4 degree for a long time ( for example, several months :) ) without any change ( concentration, degradation ).
:? :?
by jasmina
Tue Apr 13, 2010 11:07 am
Forum: Cell Biology
Topic: Cetylpyridinium chloride solution storage
Replies: 4
Views: 4524

Cetylpyridinium chloride solution storage

hi there!
In my experiments, i used Cetylpyridinium chloride to treat my cells, i bought it from sigma http://www.sigmaaldrich.com/catalog/Pro ... KEY&F=SPEC, and dissolved it in autoclaved DDW, but i am not sure about its storage conditions, now i stored solution at 4 degree, i am wondering how long can it keep stable at 4 degree?
by jasmina
Sat Apr 10, 2010 5:59 am
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 3014

Re:

how long do you usually block for? Maybe 1 hour block is just not enough? Though I must confess, I've never seen an anomaly like yours, I don't understand why BSA would preferentially bind to where the lanes are and not in between my antibody is phospho-p38 MAPK kinase(Thr180/Tyr182) antibody. Usua...
by jasmina
Fri Apr 09, 2010 6:29 am
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 3014

Re: western results

I did detection again today, using same antibody, but varied protocol. yesterday, i used 5% BSA blocking, and blocked 1hr, then 1st antibody incubation overnight at 4 degree. today, i used 5% skim milk blocking, blocked overnight at 4 degree then incubated with 1st antibody 1hr at room temperature. ...
by jasmina
Fri Apr 09, 2010 12:59 am
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 3014

Re:

JackBean wrote:what are the big black spots?


I have no idea about it, previous, when i detected this antibody, expect aim bands, background is clear. so i do not know what happened this time. :?
by jasmina
Thu Apr 08, 2010 9:12 am
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 3014

western results

hi there!
Today after detection, i got result as following (attachment). I think there are white bands which size is little bigger than 42KDa, and between each bands, i can see blackspot, i never got result like this before. confusing :?:
by jasmina
Wed Apr 07, 2010 1:13 am
Forum: Molecular Biology
Topic: PCR aim band size
Replies: 5
Views: 5282

Thank you ! O(∩_∩)O~ i get it. first download aim gene mRNA sequence ( select genus) ,then blast 2 primers and mRNA sequence with blastn-suite respectively, after get annealing site, calculate band size, right?
by jasmina
Tue Apr 06, 2010 8:58 am
Forum: Molecular Biology
Topic: PCR aim band size
Replies: 5
Views: 5282

Re:

just download the sequence of your gene and look, where the primers anneal and from that calculate the size of your amplicon. E.g. if your fw primer anneals to +25 nt and rev primer anneal to +450, than your amplicon will have 450-25 = ~425 nt but in most cases, primers are small, sometimes they ca...