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by aviprem
Mon Aug 04, 2008 1:16 pm
Forum: Molecular Biology
Topic: PCR for long DNA fragment
Replies: 4
Views: 7394

Re: PCR for long DNA fragment

For amplification of long templates, use specific long PCR amplification polymerases with proof reading activity, like Expand Hi Fi Polymerase (Roche). If you have trouble in amplifying the gene as a 6 kb single fragment , try designing primers to amplify gene as fragments ( eg two 3 Kb fragments or...