Search found 4 matches

by Hannah2008
Mon Feb 07, 2011 7:52 pm
Forum: Molecular Biology
Topic: 2D gel IEF equilibration buffer
Replies: 2
Views: 1744

I'm not sure why, but these references say to not leave urea buffers at room temperature for extended periods of time.
http://onlinelibrary.wiley.com/doi/10.1 ... 75.oth/pdf
www.hoeferinc.com/downloads/IEF100_IMA0.pdf
by Hannah2008
Mon Feb 07, 2011 7:49 pm
Forum: Molecular Biology
Topic: Sense effect of RNAi
Replies: 9
Views: 6826

If you are introducing a sense strand of mRNA into the cell, what would it bind to form a duplex, since the endogenous mRNA would also be in the sense direction?
Unless there is double stranded contamination, which is one explanation...
by Hannah2008
Mon Feb 07, 2011 7:41 pm
Forum: Molecular Biology
Topic: 2D gel IEF equilibration buffer
Replies: 2
Views: 1744

2D gel IEF equilibration buffer

Hi guys,
I left my isoelectric focusing (IEF) equilibration buffer out at RT over the weekend. Is it still ok to use?
Thanks,
Hannah
by Hannah2008
Thu Feb 03, 2011 3:53 pm
Forum: Molecular Biology
Topic: Sense effect of RNAi
Replies: 9
Views: 6826

Sense effect of RNAi

Does anyone know the basis for why sense RNA has been shown to be effective at knocking down transcription as antisense? Thanks,
Hannah