Search found 2 matches

by nesma7
Wed Jul 23, 2008 12:35 am
Forum: Molecular Biology
Topic: gene instability or gene product toxicity?
Replies: 0
Views: 1085

gene instability or gene product toxicity?

I synthesized a gene of 192 bp coding for miniproinsulin by a gene synthesis company. the gene was sent to me in pCR4-TOPO vector cloning that vector in DH5α or Top 10 E.coli was not sucessful {didn't give the expected size of the plasmid & did not contain my restriction sites ( BamHI & Hind...
by nesma7
Thu Jun 26, 2008 10:45 pm
Forum: Molecular Biology
Topic: cloning of gene in pcr4-topo vector or PQE 30 not successful
Replies: 1
Views: 3944

cloning of gene in pcr4-topo vector or PQE 30 not successful

I synthesized a gene of 192 bp coding for miniproinsulin by a gene synthesis company. After recieving the gene I tried the cloning, but I faced a problem. Here is a brief sequence of events since I started to use the gene: - the gene was sent to me in pCR4-TOPO vector, I tried to propagate it in DH5...