Search found 10 matches

by cnite
Fri Jun 06, 2008 3:21 pm
Forum: Molecular Biology
Topic: DNA Replication-Polymerase/Primer
Replies: 3
Views: 4717

Re: DNA Replication-Polymerase/Primer

For some reason I am having a hard time completely understand exactly why a primer is needed before DNA polymerase can work.. Because it ensures self-correcting mechanism by 3'->5' DNAP endonuclease activity can work. also I am not a hundred percent sure how DNA polymerase actually works. Can anyon...
by cnite
Sat May 24, 2008 8:11 pm
Forum: Molecular Biology
Topic: GENE ACTIVATION
Replies: 1
Views: 2198

Re: GENE ACTIVATION

knsprasad wrote:can any one give me example for cell signaling
gene activation


For example, cortisol. Activates gluconeogenesis genes and suppresses some immune system genes.
Actually, any hormone is an intercellular message whose ultimate effect is gene activation/suppression.
by cnite
Thu May 15, 2008 10:35 pm
Forum: Molecular Biology
Topic: Insertional Sequences
Replies: 4
Views: 2575

Cat, thanks
by cnite
Thu May 15, 2008 10:33 pm
Forum: Molecular Biology
Topic: DNA Transcription
Replies: 4
Views: 7900

Warning: Spoiler!

q1:

You go here
http://www.vivo.colostate.edu/molkit/tr ... index.html
and find that first direct ORF 5'-AAGATTAGTAGTACGACAATC-3' gives you KISS
Thus, you conclude that the protein is most probably hemoglobin which is found in all tissues and kisses them gently!

The same for q2
by cnite
Tue May 13, 2008 1:08 pm
Forum: Molecular Biology
Topic: Insertional Sequences
Replies: 4
Views: 2575

Re:

Cat wrote:Yes they do.


Thanks for answer. But may you explain/give link to IS map? In none of study books (MBoC4, Genomes 3, Genes 8, Human Mol. Genetics, Genetics in Medicine ) I found detailed example of map of ISes.
by cnite
Sun May 11, 2008 11:51 pm
Forum: Molecular Biology
Topic: Dispersed non-processed pseudogenes
Replies: 0
Views: 1173

Dispersed non-processed pseudogenes

Hello!

How do non-processed pseudogenes disperse throughout different chromosomes? What is a model mechanism for this?
by cnite
Sun May 11, 2008 4:04 pm
Forum: Molecular Biology
Topic: Insertional Sequences
Replies: 4
Views: 2575

Insertional Sequences

Hi All

Do ISes have promoter? If no, how do they manage to get their transposase transcribed? By chance alone?
by cnite
Sun May 11, 2008 3:58 pm
Forum: Molecular Biology
Topic: NCBI ORF Finder Problem
Replies: 2
Views: 4684

Thanks.
It's cool, but still I don't see how to make it recognize other start codons than atg...
by cnite
Sun May 11, 2008 12:35 am
Forum: Molecular Biology
Topic: NCBI ORF Finder Problem
Replies: 2
Views: 4684

NCBI ORF Finder Problem

Hello, all! Question #1: I don't understand how to make NCBI ORF Finder to react on another than ATG start codon. I set Table 11 (Bacterial code) (m) and feed it with FASTA code of MS2 bacteriophage (m) This phage has ORF on position 135 starting with GUG. In the Table 11 this codon is checked with ...
by cnite
Tue May 06, 2008 3:39 pm
Forum: General Discussion
Topic: Biology Problems
Replies: 0
Views: 1067

Biology Problems

Hello!

I remember there were questions from previous Olympiads on the site but cannot find them now. Please, give the links if they are still alive.

Except, if there are other sources of biology questions (and answers), please, post here.

Thanks