Search found 3 matches

by ripplemm
Thu May 08, 2008 12:42 am
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 4767

Re: problem with cloning using pTYB2

There is something I did not say clearly in my first post. I am doing cloning for two different proteins separately. One is 300 bp, the other is 1 kb. pTYB2 is 7.4 kb. Here is a picture for my ligation reactions. pTYB2 is the original vector, I was using it as a marker on this gel. from left to the ...
by ripplemm
Thu May 08, 2008 12:25 am
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 4767

Re: problem with cloning using pTYB2

Thanks a lot blcr11! I actually found that post by googling for pTYB2. Because it seemed that I have different issues, I thought it would be a good idea to ask for suggestions here. To answer your questions, (1). My PCR product should be double digested well. I drew my conclusion based on the self-l...
by ripplemm
Mon May 05, 2008 8:57 pm
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 4767

problem with cloning using pTYB2

I am trying to clone a 300 bp gene into pTYB2 vector (7.4kb)for later protein purification method by IMPACT-CN. NdeI (producing sticky end) and SmaI (blunt end) restriction sites were constructed into primers corresponding to the N- and C- termini of the gene and incorporated by PCR amplification. T...