Search found 8 matches

by pguo
Mon Nov 24, 2008 6:44 pm
Forum: Molecular Biology
Topic: DNA microchip techniques
Replies: 0
Views: 2432

DNA microchip techniques

Source: BioInfo website: m What is a DNA microchip? Scientists know that a mutation - or alteration - in a particular gene's DNA often results in a certain disease. However, it can be very difficult to develop a test to detect these mutations, because most large genes have many regions where mutatio...
by pguo
Fri Sep 19, 2008 4:14 am
Forum: Molecular Biology
Topic: clonıng problem...
Replies: 3
Views: 2920

Re: clonıng problem...

I think there are some good protocols and troubleshooting you can refer on the topic of molecular cloning over there.
Molecular cloning and more:
http://www.e-biotek.com/index.php?optio ... &Itemid=54
by pguo
Fri Sep 19, 2008 4:11 am
Forum: Molecular Biology
Topic: PCR Elisa and PCR SSCP
Replies: 0
Views: 1936

PCR Elisa and PCR SSCP

I found it's very difficult to do PCR Elisa and PCR-SSCP. Here are some protocols of PCR variants I found. m The PCR-SSCP has been using to probe point mutation, but there are not a lot of publications I can refer. I wonder if there is a method to enhance the signal because the bands are sometimes t...
by pguo
Thu Sep 04, 2008 7:11 am
Forum: Biology Movies
Topic: Molecular biology video sharing
Replies: 1
Views: 6934

Molecular biology video sharing

I found a bio video with a lot of lectures, animations, and movies on stem cell research, biochemistry and biology. Media players are not requested for the videos. The website link is as follows.
http://www.e-biotek.com/index.php?optio ... &Itemid=73
by pguo
Thu Jun 19, 2008 9:28 pm
Forum: Molecular Biology
Topic: protocol
Replies: 1
Views: 1596

I hope you can find the exact protocol for sequencing over here. Good luck.
http://www.lab-manual.com/lm_243.htm
by pguo
Wed May 14, 2008 7:56 pm
Forum: Cell Biology
Topic: Can I culture HESCs without feeder cell layer?
Replies: 1
Views: 2694

Can I culture HESCs without feeder cell layer?

Hi,friends, I have been culturing human embryonic stem cell lines H1 and H9, which were purchased from national stem cell center. I co-culture the cell line with MEF according to the protocol ( m ). I wonder if I can culture them without the feeder cell layer. You are very appreciated if you can let...
by pguo
Thu May 08, 2008 5:31 pm
Forum: Molecular Biology
Topic: sequence of steps for PCR, etc
Replies: 3
Views: 4395

Re: sequence of steps for PCR, etc

I think you are doing something right now, so it's urgent to know the protocols for your experiments. am I right? You can find PCR and its related protocols over here m There is another place you can find DNA, RNA, protein and their related, such as molecular cloning background and protocols. m In a...
by pguo
Mon May 05, 2008 8:34 am
Forum: Molecular Biology
Topic: Functional relationship between Proteins and DNA
Replies: 2
Views: 2094

I don't understand your question very well. Here are some protocols and related to your topic. I hope it will be helpful. m Chromatin Immunoprecipitation (ChIP) Gel Mobility Shift Assay Filter Binding (1) (2) DNase Footprinting (1) (2) DMS Footprinting Genetic analysis X-ray crystallography Methylat...